Kubotsu K, Goto S, Fujita M, Tuchiya H, Kida M, Takano S, Matsuura S, Sakurabayashi I
Osaka Research Laboratory, Wako Pure Chemical Industries, Ltd., Japan.
Clin Chem. 1992 Jun;38(6):808-12.
We developed automated homogeneous immunoassays, based on immunolysis of liposomes, for measuring phenytoin, phenobarbital, and carbamazepine from serum. Liposome lysis was detected spectrophotometrically from entrapped glucose-6-phosphate dehydrogenase activity. The procedure was fully automated on a routine automated clinical analyzer. Within-run, between-run, dilution, and recovery tests showed good accuracies and reproducibilities. Bilirubin, hemoglobin, triglycerides, and Intrafat did not affect assay results. The results obtained by liposome immunoassays for phenytoin, phenobarbital, and carbamazepine correlated well with those obtained by enzyme-multiplied immunoassay (Syva EMIT) kits (r = 0.995, 0.986, and 0.988, respectively) and fluorescence polarization immunoassay (Abbott TDx) kits (r = 0.990, 0.991, and 0.975, respectively). The proposed method should be useful for monitoring anticonvulsant drug concentrations in blood.
我们开发了基于脂质体免疫溶解的自动化均相免疫分析法，用于测定血清中的苯妥英、苯巴比妥和卡马西平。通过包封的葡萄糖-6-磷酸脱氢酶活性，采用分光光度法检测脂质体裂解。该方法在常规自动化临床分析仪上实现了完全自动化。批内、批间、稀释和回收试验显示出良好的准确性和重现性。胆红素、血红蛋白、甘油三酯和脂肪乳剂不影响测定结果。脂质体免疫分析法测定苯妥英、苯巴比妥和卡马西平的结果与酶放大免疫分析法（Syva EMIT）试剂盒（r分别为0.995、0.986和0.988）以及荧光偏振免疫分析法（Abbott TDx）试剂盒（r分别为0.990、0.991和0.975）的结果相关性良好。所提出的方法对于监测血液中抗惊厥药物浓度应是有用的。
