Enzyme immunoassay of phenobarbital, phenytoin, primidone, carbamazepine and theophylline with the Abbott VP Bichromatic Analyzer.

The enzyme multiplied immunoassay technique (EMIT) for the assay of phenobarbital, phenytoin, primidone, carbamazepine and theophylline was adapted to the Abbott VP Bichromatic Analyzer. Reagent consumption is reduced by the use of a stable working dilution of EMIT reagent B in EMIT buffer. Correlation with the standard EMIT method is excellent. This procedure greatly increases the maximum number of tests per kit. The method is an accurate and economical alternative to the standard EMIT method.