Thadikkaran Lynne, Crettaz David, Siegenthaler Michèle A, Gallot Denis, Sapin Vincent, Iozzo Renato V, Queloz Pierre-Alain, Schneider Philippe, Tissot Jean-Daniel
Service Régional Vaudois de Transfusion sanguine, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.
Clin Chim Acta. 2005 Oct;360(1-2):27-36. doi: 10.1016/j.cccn.2005.04.018.
The presence and integrity of amniotic fluid is fundamental for the normal development of the human fetus during pregnancy. Its production rate changes throughout pregnancy and is mainly related to the functions of the different fetal, placental and amniotic compartments. Premature rupture of the membranes (PROM) occurs in about 5% of deliveries, with complications such as infection and preterm birth. The management of patients with PROM, regardless of gestational age, remains controversial, and it is therefore important to develop new biological tests in order to achieve accurate diagnoses by identifying the presence of specific amniotic fluid markers in vaginal environment. We recently showed the usefulness of amniotic fluid proteomics in identifying a series of peptides that were absent from the corresponding maternal plasma. Several peptides corresponded to fragments of plasma proteins. Two peptides, absent from plasma samples of pregnant women, were identified in amniotic fluid. They corresponded to the COOH-terminal parts of perlecan (SwissProt: P98160) and of agrin (SwissProt: O00468) protein cores, two major heparan sulfate proteoglycans of basement membranes. In this review we will discuss modern proteomic strategies that may improve the laboratory assessment of PROM, and will focus on some of the biochemical characteristics of agrin and perlecan fragments identified in amniotic fluid.
羊水的存在及其完整性对于人类胎儿在孕期的正常发育至关重要。其生成速率在整个孕期会发生变化，并且主要与胎儿、胎盘和羊膜腔不同部分的功能相关。胎膜早破（PROM）约发生于5%的分娩过程中，会引发感染和早产等并发症。无论孕周如何，胎膜早破患者的管理仍存在争议，因此开发新的生物学检测方法以通过识别阴道环境中特定羊水标志物的存在来实现准确诊断非常重要。我们最近表明羊水蛋白质组学在识别一系列相应母体血浆中不存在的肽段方面具有实用性。几种肽段对应于血浆蛋白的片段。在羊水中鉴定出两种在孕妇血浆样本中不存在的肽段。它们对应于基底膜的两种主要硫酸乙酰肝素蛋白聚糖——基底膜聚糖（SwissProt：P98160）和集聚蛋白（SwissProt：O00468）蛋白核心的COOH末端部分。在本综述中，我们将讨论可能改善胎膜早破实验室评估的现代蛋白质组学策略，并将重点关注在羊水中鉴定出的集聚蛋白和基底膜聚糖片段的一些生化特性。
