Department of Chemistry and Chemical Technologies, University of Calabria, Via P. Bucci, Cubo12/D, I-87036, Arcavacata di Rende (CS), Italy.
Unit of Hematology for Rare Diseases of Blood and Blood-forming Organs, Regional Reference, Laboratory of Rare Diseases Molecular Diagnosis, Palermo, Italy.
Sci Rep. 2018 Jul 20;8(1):10973. doi: 10.1038/s41598-018-29384-9.
Coelomic fluid (CF) is the earliest dynamic and complex fluid of the gestational sac. CF contains maternal cells and proteins produced by embryonic cells, tissues and excretions. The biochemical composition of CF is modified throughout the first trimester of pregnancy and its protein profile reflects both physiological/pathological changes affecting the embryo and mother. Identification of variations in the balance of proteins might indicate particular types of pathologies, or ascertain specific genetic disorders. A platform utilizing protein enrichment procedures coupled with shotgun identification and iTRAQ differentiation provided the identification and quantitation of 88 unique embryonic proteins. It is relevant to note that chromosome X protein CXorf23 was found suggesting the embryo sex. Foetal sex was determined by Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR) on coelomic cells, foetal tissues and maternal white blood cells, with a 100% concordance rate between iTRAQ-MS/MS and QF-PCR data. The functional associations among the identified proteins were investigated using STRING database. Open Targets Platform showed as significant the following therapeutic areas: nervous, respiratory, eye and head system disease.
羊膜腔液（CF）是妊娠囊中最早出现的动态且复杂的液体。CF 中含有母体细胞和胚胎细胞、组织和分泌物产生的蛋白质。CF 的生化成分在整个妊娠早期发生变化，其蛋白质谱反映了影响胚胎和母体的生理/病理变化。蛋白质平衡的变化可能表明存在特定类型的病理，或确定特定的遗传疾病。一种利用蛋白质富集程序结合鸟枪法鉴定和 iTRAQ 差异分析的平台，提供了 88 种独特胚胎蛋白的鉴定和定量。值得注意的是，发现了 X 染色体蛋白 CXorf23，提示胚胎的性别。通过对羊膜腔细胞、胎儿组织和母体白细胞进行定量荧光聚合酶链反应（QF-PCR）来确定胎儿性别，iTRAQ-MS/MS 和 QF-PCR 数据之间的符合率为 100%。使用 STRING 数据库研究了鉴定出的蛋白质之间的功能关联。Open Targets 平台显示以下治疗领域具有显著意义：神经、呼吸、眼和头部系统疾病。
