Tsai Ian-Lih, Lee Fan-Pin, Wu Chin-Chung, Duh Chang-Yih, Ishikawa Tsutomu, Chen Jih-Jung, Chen Yu-Chang, Seki Hiroko, Chen Ih-Sheng
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
Planta Med. 2005 Jun;71(6):535-42. doi: 10.1055/s-2005-864155.
Three new cyclobutanoid amides with trans-trans-trans configurations, piperarborenine C, piperarborenine D and piperarborenine E, and a new furanoid lignan, (+)-arborone, together with twelve known compounds, were isolated from the stems of Piper arborescens. The structures of these new compounds were determined by means of spectral analyses. Piperarborenine C, (+)-diayangambin, piplartine, piperolactam B, piperolactam C, aristolactam BIII, goniothalactam, and methyl trans-3,4,5-trimethoxycinnamate possessed anti-platelet aggregation activity in vitro. Among them, piplartine showed the most potent anti-platelet aggregation activity induced by collagen and showed an IC50 value of 21.5 microM. Piperarborenines A - E, piperarborenine, aristololactam BIII and goniothalactam showed significant cytotoxic activity (IC50 values < 4 microg/mL) against P-388, HT-29 and A549 cell lines in vitro.
从乔木胡椒的茎中分离出三种具有反-反-反构型的新环丁烷酰胺类化合物，即胡椒乔木宁C、胡椒乔木宁D和胡椒乔木宁E，以及一种新的呋喃类木脂素，(+)-乔木酮，同时还分离出12种已知化合物。这些新化合物的结构通过光谱分析确定。胡椒乔木宁C、(+)-二亚杨安宾、荜茇宁、胡椒内酰胺B、胡椒内酰胺C、马兜铃内酰胺BIII、鹅掌楸内酰胺和反式-3,4,5-三甲氧基肉桂酸甲酯在体外具有抗血小板聚集活性。其中，荜茇宁在体外对胶原诱导的抗血小板聚集活性最强，IC50值为21.5微摩尔/升。胡椒乔木宁A-E、胡椒乔木宁、马兜铃内酰胺BIII和鹅掌楸内酰胺在体外对P-388、HT-29和A549细胞系显示出显著的细胞毒性活性（IC50值<4微克/毫升）。
