Momeni Hamid R, Kanje Martin
Department of Cell and Organism Biology, Lund University, Helgonavägen 3 B, 223 62 Lund, Sweden.
Neuroreport. 2005 Jul 13;16(10):1065-8. doi: 10.1097/00001756-200507130-00007.
Motor neuron cell death was studied in organotypic cultures of adult spinal cord slices from the mouse. Six hours after excision, many motor neuron nuclei displayed apoptotic features including nuclear and chromatin condensation. At this time point, many motor neurons also exhibited immunoreactivity to calpain II. Both the calpain inhibitor VI and ethyleneglycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) prevented the appearance of apoptotic nuclei whereas the pan caspase inhibitor Z-VAD.fmk had no effect. The results suggest that calpain is required for apoptosis of motor neurons and that this type of apoptosis is independent of caspase activation.
在来自小鼠的成年脊髓切片的器官型培养物中研究了运动神经元细胞死亡。切除后6小时，许多运动神经元细胞核呈现出凋亡特征，包括核和染色质浓缩。在这个时间点，许多运动神经元对钙蛋白酶II也表现出免疫反应性。钙蛋白酶抑制剂VI和乙二醇双（β-氨基乙醚）N，N，N'，N'-四乙酸（EGTA）都能阻止凋亡细胞核的出现，而泛半胱天冬酶抑制剂Z-VAD.fmk则没有效果。结果表明，钙蛋白酶是运动神经元凋亡所必需的，并且这种类型的凋亡独立于半胱天冬酶激活。
