通过高速逆流色谱法从中国药用植物岩生紫菀中制备分离和纯化紫菀酮酸。

Preparative isolation and purification of rupestonic acid from the Chinese medicinal plant Artemisia rupestris L. by high-speed counter-current chromatography.

作者信息

Ma Yanming, Aisha Haji Akber, Liao Lixin, Aibai Sirafil, Zhang Tianyou, Ito Yoichiro

机构信息

Xinjiang Technical Institute of Physics & Chemistry, CAS, Urumqi 830011, China.

出版信息

J Chromatogr A. 2005 May 27;1076(1-2):198-201. doi: 10.1016/j.chroma.2005.04.040.

DOI:10.1016/j.chroma.2005.04.040

PMID:15974089

Abstract

Rupestonic acid was purified for the first time by high-speed counter-current chromatography from a dichloromethane extract of the traditional Chinese medicinal plant Artemisia rupestris L. The separation was performed in two steps with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (6:4:3.5:6.5, v/v) with 0.5% acetic acid in stationary-phase. From 200 mg of the crude extract, 27.9 mg of rupestonic acid was obtained at over 98% purity as determined by HPLC analysis, and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.

摘要

首次采用高速逆流色谱法从传统中药岩蒿的二氯甲烷提取物中纯化出岩藻酸。分离分两步进行，使用由正己烷-乙酸乙酯-甲醇-水（6:4:3.5:6.5，v/v）组成的两相溶剂系统，固定相中含有0.5%的乙酸。通过高效液相色谱分析，从200mg粗提取物中获得了27.9mg纯度超过98%的岩藻酸，其化学结构通过质谱、1H和13C核磁共振得以确认。