Li Hua-Bin, Chen Feng
Department of Botany, University of Hong Kong, Hong Kong, China.
J Chromatogr A. 2004 Aug 27;1047(2):249-53. doi: 10.1016/j.chroma.2004.07.006.
Ligusticum chuanxiong Hort has been used widely in traditional medicines for the treatment of various kinds of disorders such as cardiovascular and cerebrovascular diseases. High-speed counter-current chromatography was applied to the isolation and purification of the bioactive component chuanxiongzine from L. chuanxiong Hort. The crude chuanxiongzine was obtained by extraction with ethanol from the dried roots of L. chuanxiong Hort under sonication. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:3:7, v/v) was successfully performed yielding 11.5 mg chuanxiongzine at 96.8% purity from 300 mg of the crude extract (4.2% chuanxiongzine) with the recovery of 91% in a one-step separation.
川芎在传统医学中已被广泛用于治疗各种疾病，如心脑血管疾病。采用高速逆流色谱法从川芎中分离纯化生物活性成分川芎嗪。通过在超声处理下用乙醇从川芎干燥根中提取得到粗川芎嗪。成功地进行了制备型高速逆流色谱分离，以正己烷 - 乙酸乙酯 - 乙醇 - 水（5:5:3:7，v/v）组成的两相溶剂系统，从300mg粗提取物（4.2%川芎嗪）中一步分离得到纯度为96.8%的川芎嗪11.5mg，回收率为91%。
