高速逆流色谱一步分离纯化岩白菜素和 chrysosptertin B 从 Artemisia rupestris L.
One-step separation and purification of rupestonic acid and chrysosptertin B from Artemisia rupestris L. by high-speed counter-current chromatography.
机构信息
Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
Phytochem Anal. 2010 Mar-Apr;21(2):205-9. doi: 10.1002/pca.1169.
INTRODUCTION
Artemisia rupestris L. is a well-known traditional Chinese medicinal plant in Xinjiang. Rupestonic acid is the main active ingredient of A. rupestris L., and has been chosen as a 'marker compound' for the chemical evaluation or quality control of A. rupestris L. and its products. Although HSCCC separation method was developed before, the separation was performed with two steps using the same solvent system, which were time-consuming and waste of the solvents.
OBJECTIVE
To develop a simple HSCCC method for the separation and purification of rupestonic acid in a single run.
METHODOLOGY
The measurement of partition coefficient (K) was introduced to select the two-phase solvent system. The simple HSCCC method was established according to the selected solvent system for separation and purification of rupestonic acid. The purity of target compound was test by HPLC and the structure was identified by MS, (1)H NMR and (13)C NMR.
RESULTS
A total of 72.3 mg of rupestonic acid and 53.5 mg of chrysosptertin B with over 95% purity were yielded from 500 mg extracts of Artemisia rupestris L. in one-step separation.
CONCLUSION
The rupestonic acid was separated in a single run by HSCCC.
简介
岩蒿（Artemisia rupestris L.）是新疆一种著名的传统中药植物。岩白菜酮酸是岩蒿（Artemisia rupestris L.）的主要活性成分，已被选为岩蒿（Artemisia rupestris L.）及其产品的化学评价或质量控制的“标记化合物”。虽然之前已经开发了 HSCCC 分离方法，但该方法采用相同的溶剂系统分两步进行，耗时且浪费溶剂。
目的
开发一种简单的 HSCCC 方法，用于在单次运行中分离和纯化岩白菜酮酸。
方法
通过测量分配系数（K）来选择两相溶剂系统。根据所选溶剂系统，建立了用于分离和纯化岩白菜酮酸的简单 HSCCC 方法。通过 HPLC 测试目标化合物的纯度，并通过 MS、（1）H NMR 和（13）C NMR 鉴定其结构。
结果
从 500mg 岩蒿提取物中，一步分离得到 72.3mg 纯度超过 95%的岩白菜酮酸和 53.5mg Chrysosptertin B。
结论
通过 HSCCC 可在单次运行中分离岩白菜酮酸。
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