制备型高速逆流色谱法在从少花翠雀中分离黑种草亭碱的应用
APPLICATION OF PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF ELATINE FROM DELPHINIUM SHAWURENSE.
作者信息
Yang Yi, Gu Dongyu, Wu Hankui, Aisa Haji Akber, Zhang Tianyou, Ito Yoichiro
机构信息
Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
J Liq Chromatogr Relat Technol. 2008 Jan 1;31(19):3012-3019. doi: 10.1080/10826070802424956.
Abstract
Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high-speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.
摘要
首次采用高速逆流色谱法（HSCCC）对短距翠雀花中的刺桐碱进行了制备分离。分离采用由乙酸乙酯 - 氯仿 - 甲醇 - 水（3:0.1:2:3，v/v）组成的溶剂体系，以较低的有机相作为流动相，在800转/分钟的转速下进行。得到了72毫克纯度超过97%且回收率约为95%的刺桐碱。通过质谱（MS）和核磁共振（NMR）鉴定了其化学结构。
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