Geoffroy Marie-Claude, Salvetti Anna
INSERM U649, Nantes, France.
Curr Gene Ther. 2005 Jun;5(3):265-71. doi: 10.2174/1566523054064977.
The human parvovirus Adeno-Associated virus (AAV-2) has been classified as a Dependovirus because it requires the presence of a helper virus to achieve a productive replication cycle. Several viruses such as Adenovirus (Ad), Herpes Simplex Virus (HSV), Vaccinia virus, and human papillomaviruses (HPV) can provide the helper activities required for AAV growth. The studies on the helper activities provided by adenovirus have provided useful information not only to understand the AAV-2 biology but also to develop tools for the production of recombinant AAV particles (rAAV). This review will focus on the current knowledge about the helper activities provided by the most extensively studied helper viruses, Ad and HSV-1, and also illustrate the methods used to supply the helper functions rAAV assembly.
人细小病毒腺相关病毒（AAV-2）已被归类为依赖病毒，因为它需要辅助病毒的存在才能完成有效的复制周期。几种病毒，如腺病毒（Ad）、单纯疱疹病毒（HSV）、痘苗病毒和人乳头瘤病毒（HPV），可以提供AAV生长所需的辅助活性。对腺病毒提供的辅助活性的研究不仅为理解AAV-2生物学提供了有用信息，也为开发生产重组AAV颗粒（rAAV）的工具提供了帮助。本综述将重点关注目前关于研究最广泛的辅助病毒Ad和HSV-1所提供的辅助活性的知识，并阐述用于提供rAAV组装辅助功能的方法。
