健胎液对胚胎着床障碍小鼠子宫雌激素受体及mRNA表达的影响。
Effects of Jiantaiye on estrogen receptor and mRNA expressions in uterus of mice with embryo implantation dysfunction.
作者信息
Liu Yan-juan, Huang Guang-ying, Yang Ming-wei, Gong Ping, Zhang Ming-min, Lu Fu-er
机构信息
Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.
出版信息
Chin J Integr Med. 2005 Mar;11(1):41-4. doi: 10.1007/BF02835748.
OBJECTIVE
To explore the effect of Jiantaiye (JTY) on the expression of estrogen receptor (ER) and ER mRNA in uterus of mice with embryo implantation dysfunction.
METHODS
Embryo implantation dysfunction mouse models were induced with mifepristone and treated with JTY. All animals were sacrificed on day 8 of pregnancy. The endometrial ER and ER mRNA expressions were assessed by immunnohistochemical SP method and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTS
Area ratio and absorbency of ER in the JTY treated group's gland and stroma were higher than those of the model group, quite similar to those of the normal control's, and ER mRNA expression in treated group's uterus was significantly higher than that in the models, but it was not significantly different from the normal control.
CONCLUSION
JTY improves the endometrial development by increasing ER and ER mRNA expressions of uterus of mice with embryo implantation dysfunction.
目的
探讨健胎液（JTY）对胚胎着床功能障碍小鼠子宫雌激素受体（ER）及ER mRNA表达的影响。
方法
用米非司酮建立胚胎着床功能障碍小鼠模型，并用JTY进行治疗。所有动物在妊娠第8天处死。采用免疫组织化学SP法和半定量逆转录聚合酶链反应（RT-PCR）检测子宫内膜ER及ER mRNA表达。
结果
JTY治疗组腺体和间质中ER的面积比和吸光度高于模型组，与正常对照组相近，治疗组子宫ER mRNA表达明显高于模型组，但与正常对照组无明显差异。
结论
JTY通过增加胚胎着床功能障碍小鼠子宫ER及ER mRNA表达来改善子宫内膜发育。
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