Zhang Ming-min, He Xin-fang, Zhang Jin-jin
Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Mar;30(3):291-4.
To compare the effects and mechanisms of Bushen Antai Recipe (BAR) and its different compositions, Bushen Recipe (BSR, the Shen-nourishing part) and Huoxue Recipe (HXR, the blood-activating part) on the endometrial receptivity in mice with blastocyst implantation dysfunction (BID).
Model mice of BID induced by indomethacin were treated respectively with BAR, BSR and HXR from the first day of pregnancy, and killed on day 5 or 6. Samples of their serum and uterine were collected for detecting serum estradiol (E2), progesterone (P) contents using radioimmunoassay; and endometrial expression levels of their receptors, ER and PR, using immunohistochemistry.
Serum levels of E2 and P, and endometrial expression of ER and PR increased significantly on day 6 in mice after treated by BAR, but in those treated by BSR and HXR, the improvements were significantly lesser.
BAR, by combined application of both Shen-nourishing and blood-activating methods, could impact E2, P, ER and PR to improve the endometrial receptivity to a higher extent in BID mice so as to promote embryo implantation.
比较补肾安胎方（BAR）及其不同组分补肾方（BSR，补肾部分）和活血方（HXR，活血部分）对胚泡着床功能障碍（BID）小鼠子宫内膜容受性的影响及其作用机制。
以吲哚美辛诱导建立BID模型小鼠，于妊娠第1天起分别给予BAR、BSR和HXR干预，于妊娠第5天或第6天处死小鼠，采集血清和子宫样本，采用放射免疫法检测血清雌二醇（E2）、孕酮（P）含量；采用免疫组织化学法检测子宫内膜雌激素受体（ER）、孕激素受体（PR）表达水平。
给予BAR治疗的小鼠在妊娠第6天血清E2、P水平及子宫内膜ER、PR表达均显著升高，而给予BSR和HXR治疗的小鼠上述指标改善程度明显较小。
补肾安胎方通过补肾与活血方法联用，可影响E2、P、ER和PR，在更大程度上改善BID小鼠子宫内膜容受性，从而促进胚胎着床。
