Zhou Hui-Fang, Li Ai-Ping, Tan Yong
Nanjing of Traditional Chinese Medicine University, Nanjing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Jul;29(7):628-31.
To observe the effects of Bushen Zhuyun Recipe (BZR) on protein expressions of estrogen receptor (ER), progesterone receptor (PR) and integrin alpha5 and beta3 in endometrium of rats at the implantation stage, for exploring the possible mechanism of the recipe in treating luteal phase defect (LPD) infertility.
Female SD rats were randomly divided into 6 groups, the blank group, the model group, the WM group treated by Western medicine, and the three BZR groups treated by low-, middle- and high-dose BZR respectively. Rats were made to pregnancy and sacrificed at the implantation stage, their middle segment of uterus, about 1 cm in length was gotten for detecting the protein expressions by Western blot. Results The protein expressions of endometrial ER and PR were significantly higher, while those of integrin alpha5 and beta3 were significantly lower than those of the control group (P < 0.05). The protein expressions of endometrial ER and PR were significantly lower, but those of integrin alpha5 and integrin beta3 were higher in rats treated by middle- and high- dose BZR than those in model rats (P < 0.05).
BZR can raise the receptivity of rats' endometrium through down-regulating the expressions of ER, PR and increasing the protein expression of integrin alpha5 and beta3 in endometrium and thus to enhance the pregnant rate.
观察补肾助孕方对着床期大鼠子宫内膜雌激素受体（ER）、孕激素受体（PR）及整合素α5和β3蛋白表达的影响，探讨该方治疗黄体功能不全（LPD）性不孕症的可能机制。
将雌性SD大鼠随机分为6组，即空白组、模型组、西药治疗组及低、中、高剂量补肾助孕方组。使大鼠受孕并在着床期处死，取其子宫中段约1 cm长组织，采用蛋白质印迹法检测蛋白表达。结果与对照组相比，模型组大鼠子宫内膜ER和PR蛋白表达显著升高，整合素α5和β3蛋白表达显著降低（P<0.05）。中、高剂量补肾助孕方组大鼠子宫内膜ER和PR蛋白表达显著低于模型组，而整合素α5和整合素β3蛋白表达高于模型组（P<0.05）。
补肾助孕方可通过下调ER、PR表达，上调子宫内膜整合素α5和β3蛋白表达，提高大鼠子宫内膜容受性，从而提高受孕率。
