采用酶联免疫吸附测定（ELISA）技术对沙特阿拉伯绵羊和山羊的口疮抗体进行血清学监测。

Sero-surveillance for orf antibodies in sheep and goats in Saudi Arabia employing the ELISA technique.

作者信息

Housawi F M, Abu Elzein E M, al Afaleq A I, Amin M M

机构信息

College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Ahsa, Saudi Arabia.

出版信息

J Comp Pathol. 1992 Feb;106(2):153-8. doi: 10.1016/0021-9975(92)90044-u.

DOI:10.1016/0021-9975(92)90044-u

PMID:1597532

Abstract

A serological survey was conducted on sheep and goats in Saudi Arabia to detect humoral antibodies against the orf virus. The ELISA technique was superior to the AGID and CFT in detecting such antibodies. Of the 239 abattoir serum samples examined, 60 per cent had orf antibodies by ELISA while 94.8 per cent of the convalescent sera had antibodies detectable by ELISA. With the lack of vaccination and the reports that the clinical disease is commonly seen in different parts of the Kingdom, it may be that orf is becoming enzootic in this country. Recommendations for implementing a satisfactory vaccination regimen are given.

摘要

在沙特阿拉伯对绵羊和山羊进行了一项血清学调查，以检测抗羊口疮病毒的体液抗体。酶联免疫吸附测定（ELISA）技术在检测此类抗体方面优于琼脂凝胶免疫扩散（AGID）和补体结合试验（CFT）。在所检测的239份屠宰场血清样本中，60%通过ELISA检测出有羊口疮抗体，而94.8%的康复期血清通过ELISA可检测到抗体。由于缺乏疫苗接种，且有报告称该国不同地区普遍可见临床病例，羊口疮可能正在该国成为地方病。文中给出了实施令人满意的疫苗接种方案的建议。