Azwai S M, Carter S D, Woldehiwet Z
Department of Veterinary Pathology, University of Liverpool, UK.
Vet Microbiol. 1995 Nov;47(1-2):119-31. doi: 10.1016/0378-1135(95)00055-f.
An enzyme-linked immunosorbent assay (ELISA) was developed together with a western blotting technique for the detection of total and specific IgG and IgM antibodies to the contagious ecthyma (orf) virus in camel (Camelus dromedarius) sera and for identifying the seroreactive antigens of the virus. An outbreak of generalised contagious ecthyma in camels was diagnosed for the first time in Libya; the seropositivity rate in a herd with clinically affected camels was 37.9% (and was related to clinical signs) and in apparently normal herds was 0% to 6.8%. Two viral antigenic determinants (22 and 40 kDa) were shared by the western blotting patterns of all the positive camel sera tested, another viral antigenic component of 28 kDa was shared by the positive sera with high ELISA titres. Very close similarity was seen with the western blot of orf-positive sheep sera. It is considered that the ELISA technique was valid for orf serodiagnosis in the camel and could be usefully applied to other species at risk of orf infection.
开发了一种酶联免疫吸附测定（ELISA）以及蛋白质印迹技术，用于检测骆驼（单峰驼）血清中针对传染性脓疱性皮炎（羊口疮）病毒的总IgG和IgM抗体以及特异性IgG和IgM抗体，并鉴定该病毒的血清反应性抗原。在利比亚首次诊断出骆驼群中爆发全身性传染性脓疱性皮炎；在有临床症状的骆驼群中血清阳性率为37.9%（且与临床症状有关），在明显正常的骆驼群中为0%至6.8%。所有检测的阳性骆驼血清的蛋白质印迹图谱共有两个病毒抗原决定簇（22和40 kDa），另一个28 kDa的病毒抗原成分存在于ELISA滴度高的阳性血清中。与羊口疮阳性绵羊血清的蛋白质印迹图谱有非常相似之处。认为ELISA技术对骆驼的羊口疮血清学诊断有效，并且可有效地应用于有羊口疮感染风险的其他物种。
