Milleville M, Cousin C, Boulanger P
C R Seances Soc Biol Fil. 1979;173(2):288-302.
The two-dimensional immunoelectrophoresis has been applied to human adenovirus type-2 structural analysis. This method is both qualitative and quantitative and was used in the following studies. 1) Detection of major and minor capsid antigens. 2) Identification of viral antigenic components in the infected cell material. 3) Quantification of antigens and serological characterization of ts mutants. 4) Identification of polymeric forms of structural proteins. 5) Assembly analysis of capsid subunits. 6) Stoichiometric analysis of proteins within the virion. 7) Obtaining of monospecific sera.
二维免疫电泳已应用于人类2型腺病毒的结构分析。该方法兼具定性和定量功能，并用于以下研究：1）检测主要和次要衣壳抗原；2）鉴定感染细胞材料中的病毒抗原成分；3）对温度敏感突变体进行抗原定量和血清学特征分析；4）鉴定结构蛋白的聚合形式；5）衣壳亚基的组装分析；6）病毒粒子内蛋白质的化学计量分析；7）获取单特异性血清。
