Patni N J, Dhawale S W, Aaronson S
J Bacteriol. 1977 Apr;130(1):205-11. doi: 10.1128/jb.130.1.205-211.1977.
Chlamydomonas reinhardi, cultured under normal growth conditions, secreted significant amounts of protein and carbohydrates but not lipids or nucleic acids. A fivefold increase in light intensity led to a tenfold increase in secreted protein and carbohydrate. Among the proteins secreted was acid phosphatase with a pH optimum at 4.8 like the enzyme in the cells. Phosphorus depleted algae grown on minimal orthophosphate contained and secreted both acid and alkaline phosphatase. The pH optimum of the intracellular alkaline phosphatase was 9.2. When phosphorus-depleted cells were grown with increasing orthophosphate, intra- and extracellular alkaline phosphatase was almost completely repressed and intra- and extracellular acid phosphatase was partially repressed. Extracellular acid and alkaline phosphatase increased with the age of the culture. Electrophoresis indicated only one acid and one alkaline phosphatase in phosphorus-satisfied and phosphorus-depleted cells. Chlamydomonas cells suspended in an inorganic salt solution secreted only acid phosphatase; the absence of any extr-cellular cytoplasmic marker enzyme indicated that there was little, if any, autolysis to account for the extracellular acid enzyme. Phosphorus-depleted cells were able to grow on organic phosphates as the sole source of orthophosphate. Ribose-5-phosphate was the best for cell multiplication, and its utility was shown to be due to the cell's ability to use the ribose as well as the orthophosphatase for cell multiplication.
莱茵衣藻在正常生长条件下培养时，会分泌大量蛋白质和碳水化合物，但不分泌脂质或核酸。光照强度增加五倍会导致分泌的蛋白质和碳水化合物增加十倍。分泌的蛋白质中有酸性磷酸酶，其最适pH值为4.8，与细胞内的该酶相同。在最低限度的正磷酸盐上生长的缺磷藻类含有并分泌酸性和碱性磷酸酶。细胞内碱性磷酸酶的最适pH值为9.2。当缺磷细胞在正磷酸盐增加的情况下生长时，细胞内和细胞外的碱性磷酸酶几乎完全受到抑制，细胞内和细胞外的酸性磷酸酶则部分受到抑制。细胞外酸性和碱性磷酸酶随着培养时间的延长而增加。电泳显示，在磷充足和缺磷的细胞中分别只有一种酸性磷酸酶和一种碱性磷酸酶。悬浮在无机盐溶液中的莱茵衣藻细胞只分泌酸性磷酸酶；缺乏任何细胞外细胞质标记酶表明，即使有自溶现象，也很少能解释细胞外酸性酶的存在。缺磷细胞能够以有机磷酸盐作为正磷酸盐的唯一来源生长。5-磷酸核糖最有利于细胞增殖，其效用表明是由于细胞能够利用核糖以及正磷酸酶进行细胞增殖。
