• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜热放线菌Tsilinsky中磷酸盐的利用及磷酸酶的组成型合成

Phosphate utilization and constitutive synthesis of phosphatases in Thermoactinomyces vulgaris Tsilinsky.

作者信息

Sinha U, Singh V P

出版信息

Biochem J. 1980 Aug 15;190(2):457-60. doi: 10.1042/bj1900457.

DOI:10.1042/bj1900457
PMID:6258569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162112/
Abstract

Thermoactinomyces vulgaris utilized both organic and inorganic phosphates with equal efficiency for its growth. The specific activities of the thermophilic acid and alkaline phosphatases were found to be maximum at 1 mM concentration of each phosphate source. All the phosphatase isoenzymes (three alkaline and one acidic) were observed irrespective of the substrate source and concentration, suggesting constitutive synthesis of the enzymes. During growth and differentiation, both acid and alkaline phosphatases exhibited uniformly stable patterns of isoenzymes with slight variations in their specific activities.

摘要

普通嗜热放线菌利用有机磷酸盐和无机磷酸盐进行生长的效率相同。发现嗜热酸性和碱性磷酸酶的比活性在每种磷酸盐源浓度为1 mM时最高。无论底物来源和浓度如何，均观察到所有磷酸酶同工酶（三种碱性和一种酸性），这表明这些酶是组成型合成的。在生长和分化过程中，酸性和碱性磷酸酶均表现出同工酶模式一致稳定，但比活性略有变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7e3/1162112/b7bf43ec580f/biochemj00416-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7e3/1162112/b7bf43ec580f/biochemj00416-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7e3/1162112/b7bf43ec580f/biochemj00416-0228-a.jpg

相似文献

1
Phosphate utilization and constitutive synthesis of phosphatases in Thermoactinomyces vulgaris Tsilinsky.嗜热放线菌Tsilinsky中磷酸盐的利用及磷酸酶的组成型合成
Biochem J. 1980 Aug 15;190(2):457-60. doi: 10.1042/bj1900457.
2
Phosphatase synthesis in Klebsiella (aerobacter) aerogenes growing in continuous culture.在连续培养条件下生长的产气克雷伯菌（产气气杆菌）中磷酸酶的合成。
Biochem J. 1972 Mar;127(1):87-96. doi: 10.1042/bj1270087.
3
Role of organic phosphates in the formation of phosphatases in Aspergillus nidulans.
Indian J Exp Biol. 1975 Nov;13(6):545-7.
4
Separation and identification of Neurospora phosphatases by polyacrylamide gel electrophoresis.
Anal Biochem. 1973 Feb;51(2):516-22. doi: 10.1016/0003-2697(73)90508-3.
5
The effect of cations on the thermophilic character of alkaline phosphatase from Thermoactinomyces vulgaris.
Biochem Mol Biol Int. 1993 Mar;29(4):729-37.
6
Mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in Thermoactinomyces vulgaris.镁离子降低了嗜热放线菌中磷酸酶高温催化的阿累尼乌斯活化能。
Curr Microbiol. 2007 Sep;55(3):179-84. doi: 10.1007/s00284-006-0539-y. Epub 2007 Jul 25.
7
The cell surface associated phosphatase activity of Mycobacterium bovis BCG is not regulated by environmental inorganic phosphate.牛分枝杆菌卡介苗的细胞表面相关磷酸酶活性不受环境无机磷酸盐的调节。
FEMS Microbiol Lett. 2001 Feb 20;195(2):121-6. doi: 10.1111/j.1574-6968.2001.tb10508.x.
8
Differential expression of thermophilic phosphatases in the wild type and auxotrophic mutant strains of Thermoactinomyces vulgaris.嗜热脂肪酶在普通野生型和营养缺陷型高温放线菌中的差异表达。
Indian J Microbiol. 2007 Mar;47(1):81-5. doi: 10.1007/s12088-007-0015-9. Epub 2007 Jun 14.
9
Alkaline, acid, and neutral phosphatase activities are induced during development in Myxococcus xanthus.在黄色黏球菌发育过程中，碱性磷酸酶、酸性磷酸酶和中性磷酸酶的活性会被诱导。
J Bacteriol. 1990 May;172(5):2294-302. doi: 10.1128/jb.172.5.2294-2302.1990.
10
Production of acid and alkaline phosphatases by Myxococcus coralloides.珊瑚状粘球菌产酸性和碱性磷酸酶的研究。
Folia Microbiol (Praha). 1989;34(3):185-94. doi: 10.1007/BF02821291.

引用本文的文献

1
Differential expression of thermophilic phosphatases in the wild type and auxotrophic mutant strains of Thermoactinomyces vulgaris.嗜热脂肪酶在普通野生型和营养缺陷型高温放线菌中的差异表达。
Indian J Microbiol. 2007 Mar;47(1):81-5. doi: 10.1007/s12088-007-0015-9. Epub 2007 Jun 14.
2
Insights into different dependence of dNTP triphosphohydrolase on metal ion species from intracellular ion concentrations in Thermus thermophilus.嗜热栖热菌细胞内离子浓度对三磷酸脱氧核苷三磷酸水解酶不同金属离子依赖性的见解。
Extremophiles. 2008 Mar;12(2):217-23. doi: 10.1007/s00792-007-0118-6. Epub 2007 Nov 8.
3
Mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in Thermoactinomyces vulgaris.

本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.无机磷酸盐对大肠杆菌中磷酸酶形成的影响。
Biochim Biophys Acta. 1960 Mar 11;38:460-9. doi: 10.1016/0006-3002(60)91281-6.
3
Mutants of Escherichia coli constitutive for alkaline phosphatase.大肠杆菌碱性磷酸酶组成型突变体。
镁离子降低了嗜热放线菌中磷酸酶高温催化的阿累尼乌斯活化能。
Curr Microbiol. 2007 Sep;55(3):179-84. doi: 10.1007/s00284-006-0539-y. Epub 2007 Jul 25.
J Bacteriol. 1961 May;81(5):835-6. doi: 10.1128/jb.81.5.835-836.1961.
4
Purification and properties of an alkaline phosphatase of Bacillus licheniformis.地衣芽孢杆菌碱性磷酸酶的纯化及性质
Biochemistry. 1971 Apr 13;10(8):1364-71. doi: 10.1021/bi00784a014.
5
Effect of inorganic phosphate upon Salmonella typhimurium phosphatase activities: non-respressible alkaline phosphatase and non-inhibited acid phosphatase.无机磷酸盐对鼠伤寒沙门氏菌磷酸酶活性的影响：非阻遏性碱性磷酸酶和非抑制性酸性磷酸酶。
Biochim Biophys Acta. 1967;146(2):535-43. doi: 10.1016/0005-2744(67)90237-9.
6
Phosphatase synthesis in Klebsiella (aerobacter) aerogenes growing in continuous culture.在连续培养条件下生长的产气克雷伯菌（产气气杆菌）中磷酸酶的合成。
Biochem J. 1972 Mar;127(1):87-96. doi: 10.1042/bj1270087.
7
Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae.酿酒酵母中可阻遏酸性磷酸酶合成的隐性组成型突变的分离与鉴定。
J Bacteriol. 1975 Jun;122(3):911-22. doi: 10.1128/jb.122.3.911-922.1975.
8
Biochemical and physiological properties of alkaline phosphatases in five isolates of marine bacteria.五株海洋细菌中碱性磷酸酶的生化及生理特性
J Bacteriol. 1977 Mar;129(3):1607-12. doi: 10.1128/jb.129.3.1607-1612.1977.
9
Phosphate utilization and alkaline phosphatase activity in Anacystis nidulans (Synechococcus).集胞藻（聚球藻）中的磷酸盐利用与碱性磷酸酶活性
Arch Microbiol. 1975;102(1):23-8. doi: 10.1007/BF00428340.
10
Chemostat culture of Escherichia coli K-12 limited by the activity of alkaline phosphatase.受碱性磷酸酶活性限制的大肠杆菌K-12恒化器培养
Appl Microbiol. 1975 Aug;30(2):267-70. doi: 10.1128/am.30.2.267-270.1975.