Nesmeianova M A, Maraeva O B, Kolot M N, Kulaev I S
Biokhimiia. 1978 Oct;43(10):1783-9.
The effect of exogenous orthophosphate and mutations in regulatory genes of alkaline phosphatase on the level of nonspecific acid phosphatase was studied. The level of this enzyme as well as the level of alkaline phosphatase were shown to be regulated by exogenous orthophosphate being derepressed under phosphate starvation. The derepression of acid phosphatase is accompanied by more rapid secretion of enzyme from membranes to soluble fraction. Mutations in all the four regulatory genes decrease the level of enzyme in cells. Genes phoR and phoS, participating in regulation of alkaline phosphatase, are required for the derepression of acid phosphatase under the conditions of phosphate starvation.
研究了外源性正磷酸盐以及碱性磷酸酶调控基因突变对非特异性酸性磷酸酶水平的影响。结果表明，该酶的水平以及碱性磷酸酶的水平受外源性正磷酸盐调控，在磷酸盐饥饿条件下会发生去阻遏。酸性磷酸酶的去阻遏伴随着酶从膜向可溶性部分的更快分泌。所有四个调控基因的突变都会降低细胞中酶的水平。参与碱性磷酸酶调控的phoR和phoS基因是磷酸盐饥饿条件下酸性磷酸酶去阻遏所必需的。
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