de Boer D, Cremers F, Teertstra R, Smits L, Hille J, Smeekens S, Weisbeek P
Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, NL 3584 CH Utrecht, the Netherlands.
EMBO J. 1988 Sep;7(9):2631-35. doi: 10.1002/j.1460-2075.1988.tb03115.x.
Transgenic tomato plants that constitutively express a foreign plastocyanin gene were used to study protein transport in different tissues. Normally expression of endogenous plastocyanin genes in plants is restricted to photosynthetic tissues only, whereas this foreign plastocyanin protein is found to be present in all tissues examined. The protein is transported into the local plastids in these tissues and it is processed to the mature size. We conclude that plastids of developmentally different tissues are capable of importing precursor proteins that are normally not found in these tissues. Most likely such plastids, though functionally and morphologically differentiated, have similar or identical protein import mechanisms when compared to the chloroplasts in green tissue.
利用组成型表达外源质体蓝素基因的转基因番茄植株来研究不同组织中的蛋白质转运。正常情况下，植物内源质体蓝素基因的表达仅局限于光合组织，而这种外源质体蓝素蛋白在所有检测的组织中均有发现。该蛋白被转运到这些组织中的局部质体中，并被加工成成熟大小。我们得出结论，发育不同的组织中的质体能够导入通常在这些组织中不存在的前体蛋白。很可能这些质体虽然在功能和形态上有差异，但与绿色组织中的叶绿体相比，具有相似或相同的蛋白质导入机制。
