Coruzzi G, Broglie R, Edwards C, Chua N H
EMBO J. 1984 Aug;3(8):1671-9. doi: 10.1002/j.1460-2075.1984.tb02031.x.
We have examined the expression of a member of the multigene family encoding the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase in various tissues of pea. The rbcS gene, pPS-2.4, was characterized by DNA sequence analysis and 5' and 3' end mapping of its mRNA transcript. rbcS polypeptides were shown to be differentially present in various tissues of light- and dark-grown plants. Northern analysis shows that compared with green leaves, the level of rbcS mRNA is reduced to approximately 50% in pericarps, 8% in petals and seeds, and 1-3% in etiolated leaves, stems, and roots. 5' S1 nuclease mapping of total rbcS mRNA was used to quantitate the relative amount of pPS-2.4 gene-specific transcripts in each tissue. pPS-2.4 mRNA accounts for approximately 30-35% of total rbcS transcripts in green leaves, but only 5-10% in pericarps, 15-20% in seeds, and is below detection in petals and etiolated leaves. We conclude that the pPS-2.4 gene is expressed in a tissue-specific, light-regulated fashion and that transcriptional controls of individual rbcS genes vary.
我们研究了编码1,5-二磷酸核酮糖羧化酶小亚基（rbcS）的多基因家族成员在豌豆不同组织中的表达情况。通过DNA序列分析以及对其mRNA转录本的5'和3'末端作图，对rbcS基因pPS - 2.4进行了表征。结果表明，rbcS多肽在光照和黑暗条件下生长的植物的不同组织中存在差异。Northern分析显示，与绿叶相比，rbcS mRNA水平在果皮中降至约50%，在花瓣和种子中降至8%，在黄化叶、茎和根中降至1 - 3%。利用总rbcS mRNA的5'S1核酸酶作图来定量每个组织中pPS - 2.4基因特异性转录本的相对量。pPS - 2.4 mRNA在绿叶中约占总rbcS转录本的30 - 35%，但在果皮中仅占5 - 10%，在种子中占15 - 20%，在花瓣和黄化叶中低于检测水平。我们得出结论，pPS - 2.4基因以组织特异性、光调节的方式表达，并且各个rbcS基因的转录调控存在差异。
