Speer Runa, Wulfkuhle Julia D, Liotta Lance A, Petricoin Emanuel F
National Institutes of Health, National Cancer Institute, Center for Cancer Research, FDA-NCI Clinical Proteomics Program, Laboratory of Pathology, Bethesda, MD 20892, USA.
Curr Opin Mol Ther. 2005 Jun;7(3):240-5.
The deciphering of the human genome has elucidated our biological structural design and has generated insights into disease development and pathogenesis. At the same time, knowledge of genetic changes during disease processes has demonstrated the need to move beyond genomics towards proteomics and a systems biology approach to science. Analyzing the proteome comprises more than just a numeration of proteins. In fact, it characterizes proteins within cells in the context of their functional status and interactions in their physiological micro- and macroenvironments. As dysregulated signaling often underpins most human diseases, an overarching goal of proteomics is to profile the working state of signaling pathways, to develop 'circuit maps' of normal and diseased protein networks and identify hyperactive, defective or inoperable transduction pathways. Reverse-phase protein microarrays represent a new technology that can generate a multiplex readout of dozens of phosphorylated events simultaneously to profile the state of a signaling pathway target even after the cell is lyzed and the contents denatured.
人类基因组的解密阐明了我们的生物结构设计，并为疾病发展和发病机制提供了深刻见解。与此同时，对疾病过程中基因变化的了解表明，有必要超越基因组学，转向蛋白质组学和系统生物学的科学研究方法。分析蛋白质组不仅仅是对蛋白质进行计数。事实上，它是在细胞内蛋白质的功能状态以及它们在生理微观和宏观环境中的相互作用背景下对其进行表征。由于信号失调通常是大多数人类疾病的基础，蛋白质组学的一个总体目标是描绘信号通路的工作状态，绘制正常和患病蛋白质网络的“电路图”，并识别过度活跃、有缺陷或无法运作的转导通路。反相蛋白质微阵列代表了一种新技术，即使在细胞裂解且内容物变性后，它也能同时对数十个磷酸化事件进行多重检测，以描绘信号通路靶点的状态。
