蛋白质微阵列作为功能蛋白质组学的工具。

Protein microarrays as tools for functional proteomics.

作者信息

LaBaer Joshua, Ramachandran Niroshan

机构信息

Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.

出版信息

Curr Opin Chem Biol. 2005 Feb;9(1):14-9. doi: 10.1016/j.cbpa.2004.12.006.

DOI:10.1016/j.cbpa.2004.12.006

PMID:15701447

Abstract

Protein microarrays present an innovative and versatile approach to study protein abundance and function at an unprecedented scale. Given the chemical and structural complexity of the proteome, the development of protein microarrays has been challenging. Despite these challenges there has been a marked increase in the use of protein microarrays to map interactions of proteins with various other molecules, and to identify potential disease biomarkers, especially in the area of cancer biology. In this review, we discuss some of the promising advances made in the development and use of protein microarrays.

摘要

蛋白质微阵列提供了一种创新且通用的方法，以前所未有的规模研究蛋白质丰度和功能。鉴于蛋白质组的化学和结构复杂性，蛋白质微阵列的开发一直具有挑战性。尽管存在这些挑战，但蛋白质微阵列在绘制蛋白质与各种其他分子的相互作用以及识别潜在疾病生物标志物方面的应用显著增加，尤其是在癌症生物学领域。在这篇综述中，我们讨论了蛋白质微阵列开发和应用方面取得的一些有前景的进展。