Abramova E B, Astakhova T M, Sharova N P
Ontogenez. 2005 May-Jun;36(3):205-10.
The dynamics of the activities of 26S and 20S proteasomes in the rat liver and spleen have been studied during postnatal development from 1 to 90 days. The activities of proteasome forms both in spleen and in liver increased in adult animals as compared to one day rats. The activities of both proteasome forms in the liver did not differ significantly from those in the spleen at all stages of postnatal development. Using Western blot with monoclonal antibodies to Rpt6 subunit, we confirmed the presence of 26S proteasome in both organs at all stages of postnatal development. Studies with polyclonal antibodies to beta1i (LMP2) subunit showed the appearance of the immune subunit in the spleen by day 9 and in the liver only by day 23 of postnatal development. This result suggests the earlier formation of the spleen as an organ with immune functions.
在出生后1至90天的发育过程中，对大鼠肝脏和脾脏中26S和20S蛋白酶体的活性动态进行了研究。与1日龄大鼠相比，成年动物脾脏和肝脏中蛋白酶体形式的活性均有所增加。在出生后发育的各个阶段，肝脏中两种蛋白酶体形式的活性与脾脏中的活性均无显著差异。使用针对Rpt6亚基的单克隆抗体进行蛋白质免疫印迹分析，我们证实了在出生后发育的各个阶段，两种器官中均存在26S蛋白酶体。用针对β1i（LMP2）亚基的多克隆抗体进行研究表明，在出生后第9天脾脏中出现了免疫亚基，而在肝脏中直到出生后第23天才出现。这一结果表明脾脏作为具有免疫功能的器官形成较早。
