T细胞免疫发育研究的新方法：大鼠早期个体发育中淋巴器官形成与免疫蛋白酶体数量变化的平行研究

New approach to study of T cellular immunity development: parallel investigation of lymphoid organ formation and changes in immune proteasome amount in rat early ontogenesis.

作者信息

Sharova Natalia P, Zakharova Liudmila A, Astakhova Tatiana M, Karpova Yaroslava D, Melnikova Victoria I, Dmitrieva Svetlana B, Lyupina Yulia V, Erokhov Pavel A

机构信息

N.K. Koltsov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.

出版信息

Cell Immunol. 2009;256(1-2):47-55. doi: 10.1016/j.cellimm.2009.01.004. Epub 2009 Feb 23.

DOI:10.1016/j.cellimm.2009.01.004

PMID:19230868

Abstract

The expression pattern and distribution of proteasome immune subunits LMP7 and LMP2 in the developing rat spleen and liver as well as the periarterial lymphoid sheath formation were investigated. LMP7 and LMP2 were detected by immunoblotting in the spleen on the 21st embryonic day and during the first postnatal days in equal amounts. Their levels increased by the 8th and 18th postnatal days. Double immunofluorescent labeling the spleen cells revealed LMP7 and LMP2 in T and B lymphocytes localized in the red pulp in embryogenesis. Few T lymphocytes were discovered in periarterial zones on the 8th postnatal day. T lymphocytes filled these zones and formed lymphoid sheaths by the 18-19th day. In the liver, LMP7 and LMP2 were revealed by the 17-19th postnatal day. Immunofluorescent analysis showed their presence in hepatocytes at this period. The data suggest that T cell-mediated immune response in relation to hepatocytes is possible beginning from 18th to 19th postnatal day.

摘要

研究了蛋白酶体免疫亚基LMP7和LMP2在发育中的大鼠脾脏和肝脏中的表达模式及分布，以及动脉周围淋巴鞘的形成。通过免疫印迹法在胚胎第21天和出生后的头几天在脾脏中检测到等量的LMP7和LMP2。它们的水平在出生后第8天和第18天升高。对脾细胞进行双重免疫荧光标记显示，在胚胎发育过程中，LMP7和LMP2存在于红髓中的T淋巴细胞和B淋巴细胞中。出生后第8天，在动脉周围区域发现的T淋巴细胞很少。到出生后第18 - 19天，T淋巴细胞充满这些区域并形成淋巴鞘。在肝脏中，出生后第17 - 19天发现了LMP7和LMP2。免疫荧光分析显示此时它们存在于肝细胞中。数据表明，从出生后第18天到第19天开始，针对肝细胞的T细胞介导的免疫反应是可能的。