单序列重复区间聚合酶链反应分型作为鉴定犬小孢子菌菌株的新工具。
Inter-single-sequence-repeat-PCR typing as a new tool for identification of Microsporum canis strains.
作者信息
Cano José, Rezusta Antonio, Solé María, Gil Joaquina, Rubio María Carmen, Revillo María José, Guarro Josep
机构信息
Department de Ciencies Mediques Basiques, Universitat Rovira i Virgili, Unitat de Microbiologia, Facultat de Medicina i Ciencies de la Salut, Carrer Sant Llorenc 21, Reus, 43201 Tarragona, Spain.
出版信息
J Dermatol Sci. 2005 Jul;39(1):17-21. doi: 10.1016/j.jdermsci.2005.01.016. Epub 2005 Apr 8.
BACKGROUND
Microsporum canis is a ubiquitous dermatophyte that commonly causes human infections. Since contact with infected animals is the usual way of infection, tracing its source is an essential preventive measure.
OBJECTIVE
To type isolates of M. canis from human patients whose skin was affected, and from some animals (dogs and cats) that were closely associated to the patients.
METHODS
The inter-single-sequence-repeat-PCR (ISSR-PCR) technique has been used for typing 24 isolates of M. canis. Seventeen isolates tested were from human patients, 5 from cats and 1 from a dog
RESULTS
A total of 21 genotypes were identified. The same genotype was found infecting a patient and a cat that was living closely with him, but another member of the same family proved to be infected with two genotypes different from that. Clinical specimens from two patients had been contaminated with the same genotype, probably in the laboratory where the samples were handled.
CONCLUSION
These results demonstrate that ISSR-PCR polymorphism is a reliable method for the identification of the M. canis strains.
背景
犬小孢子菌是一种常见的皮肤癣菌，常引起人类感染。由于接触受感染动物是常见的感染途径，追踪其来源是一项重要的预防措施。
目的
对来自皮肤受影响的人类患者以及与患者密切相关的一些动物（狗和猫）的犬小孢子菌分离株进行分型。
方法
采用单序列重复区间聚合酶链反应（ISSR-PCR）技术对24株犬小孢子菌进行分型。检测的17株分离株来自人类患者，5株来自猫，1株来自狗。
结果
共鉴定出21种基因型。发现同一基因型感染了一名患者及其密切接触的一只猫，但该家庭的另一名成员被证明感染了两种不同的基因型。两名患者的临床标本被相同基因型污染，可能是在处理样本的实验室中。
结论
这些结果表明，ISSR-PCR多态性是鉴定犬小孢子菌菌株的可靠方法。
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