Shafiee Shabnam, Khosravi Ali Reza, Ashrafi Tamai Iradj
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Mycoses. 2014 Aug;57(8):507-12. doi: 10.1111/myc.12189. Epub 2014 Mar 18.
Microsporum canis is a zoophilic fungus and it is an important agent of dermatophytosis. Cats act as important reservoirs. Clinically, it is too difficult to differentiate dermatophytosis caused by various species, also this fungus loses its morphological characteristics easily because of subculture; so using of rapid and accurate laboratory techniques for identifying the dermatophytes is important, therefore, RAPD-PCR was applied for the differentiation of the isolates. In this study, 10 M. canis isolates were detected in cats, dog, human, fox and rabbit at the Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran. For running the RAPD-PCR, PCR set system and three random primers OPU 15, OPU 13 and OPA 04 were used. Then phylogenetic tree and similarity coefficient table were drawn. The results showed that there were some common bands between M. canis isolates. There were some specific bands for each isolates, as well. Our study showed, despite the typical morphology of the whole isolates, they were placed in different branches in molecular typing.
犬小孢子菌是一种亲动物性真菌，是皮肤癣菌病的重要病原体。猫是重要的储存宿主。临床上，很难区分由不同菌种引起的皮肤癣菌病，而且这种真菌由于传代培养很容易失去其形态特征；因此，使用快速准确的实验室技术来鉴定皮肤癣菌很重要，因此，随机扩增多态性DNA聚合酶链反应（RAPD-PCR）被用于分离株的鉴别。在本研究中，在德黑兰大学兽医学院真菌学研究中心的猫、狗、人、狐狸和兔子中检测到10株犬小孢子菌分离株。为进行RAPD-PCR，使用了PCR成套系统和三种随机引物OPU 15、OPU 13和OPA 04。然后绘制了系统发育树和相似系数表。结果表明，犬小孢子菌分离株之间存在一些共同条带。每个分离株也有一些特异性条带。我们的研究表明，尽管所有分离株形态典型，但在分子分型中它们被置于不同的分支。
