基于 ISSR 分型推断的土曲霉全球种群结构揭示了地理亚聚类。
Global population structure of Aspergillus terreus inferred by ISSR typing reveals geographical subclustering.
机构信息
Mycotic Diseases Branch, Center for Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30329, USA.
出版信息
BMC Microbiol. 2011 Sep 16;11:203. doi: 10.1186/1471-2180-11-203.
BACKGROUND
Aspergillus terreus causes invasive aspergillosis (IA) in immunocompromised individuals and can be the leading cause of IA in certain medical centers. We examined a large isolate collection (n = 117) for the presence of cryptic A. terreus species and employed a genome scanning method, Inter-Simple Sequence Repeat (ISSR) PCR to determine A. terreus population structure.
RESULTS
Comparative sequence analyses of the calmodulin locus revealed the presence of the recently recognized species A. alabamensis (n = 4) in this collection. Maximum parsimony, Neighbor joining, and Bayesian clustering of the ISSR data from the 113 sequence-confirmed A. terreus isolates demonstrated that one clade was composed exclusively of isolates from Europe and another clade was enriched for isolates from the US.
CONCLUSIONS
This study provides evidence of a population structure linked to geographical origin in A. terreus.
背景
土曲霉可引起免疫功能低下个体的侵袭性曲霉病（IA），并且在某些医疗中心可能是导致 IA 的主要原因。我们检查了一个大型的分离物集合（n=117），以检测隐匿性土曲霉物种的存在，并采用基因组扫描方法，即简单重复序列间 PCR（ISSR-PCR），来确定土曲霉种群结构。
结果
钙调蛋白基因座的比较序列分析显示，该集合中存在最近确认的物种 A. alabamensis（n=4）。113 个经序列确认的土曲霉分离物的 ISSR 数据的最大简约法、邻接法和贝叶斯聚类表明，一个分支完全由来自欧洲的分离物组成，另一个分支则富含来自美国的分离物。
结论
本研究提供了与土曲霉地理起源相关的种群结构的证据。
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