Schönfelder Ute, Abel Martin, Wiegand Cornelia, Klemm Dieter, Elsner Peter, Hipler Uta-Christina
Department of Dermatology, Friedrich Schiller University, 07743 Jena, Germany.
Biomaterials. 2005 Nov;26(33):6664-73. doi: 10.1016/j.biomaterials.2005.04.030.
Exudates from non-healing wounds contain elevated levels of proteolytic enzymes, like elastase from polymorphonuclear granulocytes (PMN elastase), reactive oxygen species (ROS) and reactive nitrogen species (RNS). The overproduction of proteolytic enzymes leads to reduced concentrations of growth factors and proteinase inhibitors, resulting in an imbalance between degradation and remodelling processes. Thus, the reduction of protein-degrading enzymes and scavenging of ROS and RNS seem to be suitable ways to support the healing process of chronic stagnating wounds. The aim of this study was to test selected wound dressings from different biomaterials (collagen, oxidized regenerated cellulose (ORC) and ORC/collagen mixture), regarding their antioxidative potential in vitro and their influence on the concentration and activity of PMN elastase in chronic wound fluid. Antioxidant capacity of the investigated wound dressing was determined by a pholasin-based chemiluminescent assay. PMN elastase concentration was determined by means of ELISA. Enzyme activities could be measured by a fluorescence assay. As the presented data demonstrates, all tested materials showed antioxidant capacity. In addition, the investigated materials were able to reduce the concentration and activity of PMN elastase. Beside other aspects, such as biocompatibility, biodegradability, fluid absorption and clinical effects (e.g. angiogenesis and microcirculation), the understanding of these properties may help to support the further refinement of wound dressings for improved wound healing.
不愈合伤口的渗出物中含有高水平的蛋白水解酶，如多形核粒细胞产生的弹性蛋白酶（PMN弹性蛋白酶）、活性氧（ROS）和活性氮（RNS）。蛋白水解酶的过度产生导致生长因子和蛋白酶抑制剂浓度降低，从而导致降解和重塑过程失衡。因此，减少蛋白质降解酶以及清除ROS和RNS似乎是促进慢性停滞伤口愈合过程的合适方法。本研究的目的是测试不同生物材料（胶原蛋白、氧化再生纤维素（ORC）和ORC/胶原蛋白混合物）制成的选定伤口敷料在体外的抗氧化潜力及其对慢性伤口液中PMN弹性蛋白酶浓度和活性的影响。通过基于荧光素的化学发光测定法测定所研究伤口敷料的抗氧化能力。通过ELISA测定PMN弹性蛋白酶浓度。酶活性可通过荧光测定法测量。如所呈现的数据所示，所有测试材料均显示出抗氧化能力。此外，所研究的材料能够降低PMN弹性蛋白酶的浓度和活性。除了生物相容性、生物降解性、液体吸收和临床效果（如血管生成和微循环）等其他方面外，对这些特性的了解可能有助于支持进一步改进伤口敷料以促进伤口更好地愈合。
