Jinn Tzyy-Rong, Kao Suey-Sheng, Tzen Jason T C, Wu Tzong-Yuan
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
J Biotechnol. 2005 Sep 29;119(3):255-9. doi: 10.1016/j.jbiotec.2005.04.019.
Genetic modified baculovirus (GMBV) are among the most promising alternatives to chemical insecticides. One of the deterrents to the GMBV development is the lack of simple and cost-effective methods for monitoring their efficacy and ecology in fields. Here, we demonstrate the DsRed gene from coral can serve as a convenient GMBV tracer. Insect larvae, including Trichoplusia ni, Spodoptera exigua, and Spodoptera litura, infected the GMBV containing the DsRed gene can emit red fluorescence under sun light without any prosthetic apparatus.
基因改造杆状病毒（GMBV）是化学杀虫剂最有前景的替代品之一。阻碍GMBV发展的一个因素是缺乏简单且经济高效的方法来监测其在田间的功效和生态情况。在此，我们证明来自珊瑚的DsRed基因可作为一种便捷的GMBV示踪剂。感染了含有DsRed基因的GMBV的昆虫幼虫，包括粉纹夜蛾、甜菜夜蛾和斜纹夜蛾，在阳光下无需任何辅助设备就能发出红色荧光。
