College of Chemistry and Chemical Engineering, Jishou University, Hunan 416000, PR China.
J Colloid Interface Sci. 2010 Apr 1;344(1):158-64. doi: 10.1016/j.jcis.2009.12.022. Epub 2009 Dec 16.
A novel sensitive molecularly imprinted electrochemical sensor was constructed for selective detection of clindamycin by combination of a multi-wall carbon nanotube (MWNT) layer with a thin molecularly imprinted sol-gel film. The sensor was fabricated onto Au electrode via stepwise modification of MWNT and a thin sol-gel film of molecularly imprinted polymers (MIP) by using electrodeposition method. The molecularly imprinted film displayed excellent selectivity towards clindamycin. Due to such combination, the sensor responded quickly to clindamycin. The response peak current was linear to the concentration of clindamycin in the range from 5.0 x 10(-7) mol L(-1) to 8.0 x 10(-5) mol L(-1), and the detection limit was 2.44 x 10(-8) mol L(-1). This imprinted sensor was applied to the determination of clindamycin in human urine samples successfully. These results revealed that the imprinted sensor fulfilled the selectivity, sensitivity, speed and simplicity requirements for clindamycin detection, and provided possibilities of clinical application in physiological fluids.
一种新型的敏感分子印迹电化学传感器通过将多壁碳纳米管（MWNT）层与薄的分子印迹溶胶-凝胶膜结合，用于选择性检测克林霉素。传感器通过MWNT 和分子印迹聚合物（MIP）的薄溶胶-凝胶膜的分步修饰，通过电沉积方法在 Au 电极上制备。分子印迹膜对克林霉素表现出优异的选择性。由于这种组合，传感器对克林霉素的响应很快。响应峰电流对克林霉素浓度在 5.0×10（-7）mol L（-1）至 8.0×10（-5）mol L（-1）范围内呈线性，检测限为 2.44×10（-8）mol L（-1）。该印迹传感器成功地应用于人尿样中克林霉素的测定。这些结果表明，印迹传感器满足了克林霉素检测的选择性、灵敏度、速度和简单性要求，并为生理体液中的临床应用提供了可能性。
