Zhang Zhaohui, Nie Lihua, Yao Shouzhuo
State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Talanta. 2006 Apr 15;69(2):435-42. doi: 10.1016/j.talanta.2005.10.008. Epub 2005 Nov 7.
A novel thin molecularly imprinted sol-gel film with specific recognition for cytidine was electrodeposited on the surface of piezoelectric quartz crystal (PQC) Au-electrode. In this method, a sufficiently negative potential was applied to the electrode surface to generate hydroxyl ions, which play the role of the catalyst for the hydrolysis and condensation of 3-(aminopropyl)trimethoxysilane (APTMS). The process of the preparation of the imprinted sol-gel film was investigated in detail by using the piezoelectric quartz crystal impedance (PQCI) technique and cyclic voltammetry. The thickness of the imprinted film was controlled easily by adjusting the applied potential and the deposited time. The binding capacity and the selectivity of the electrodeposited imprinted sol-gel film were also studied in detail by using PQCI, electrochemically impedance technique and capacitance technique. The electrodeposited imprinted sol-gel film exhibited high selectivity toward cytidine in comparison to interfering substances. The dissociation constant (K(d)) in the nanomolar range indicated a strong imprinted interaction existing between the electrodeposited sol-gel-imprinted film and the template cytidine.
一种对胞嘧啶具有特异性识别能力的新型薄分子印迹溶胶 - 凝胶膜被电沉积在压电石英晶体（PQC）金电极表面。在该方法中，向电极表面施加足够负的电位以产生氢氧根离子，氢氧根离子在3 - （氨丙基）三甲氧基硅烷（APTMS）的水解和缩合反应中起催化剂的作用。利用压电石英晶体阻抗（PQCI）技术和循环伏安法详细研究了印迹溶胶 - 凝胶膜的制备过程。通过调节施加的电位和沉积时间可以轻松控制印迹膜的厚度。还利用PQCI、电化学阻抗技术和电容技术详细研究了电沉积印迹溶胶 - 凝胶膜的结合能力和选择性。与干扰物质相比，电沉积的印迹溶胶 - 凝胶膜对胞嘧啶表现出高选择性。纳摩尔范围内的解离常数（K(d)）表明电沉积的溶胶 - 凝胶印迹膜与模板胞嘧啶之间存在强烈的印迹相互作用。
