Zhong R, He G, Sakai Y, McAlister V, Zhang Z, Duff J, Stiller C, Grant D
Department of Surgery, University of Western Ontario, London, Canada.
Microsurgery. 1992;13(3):126-31. doi: 10.1002/micr.1920130306.
Simultaneous liver transplantation may reduce the risk of intestinal transplant rejection. We have recently developed two new models of combined liver/intestine transplantation (LIT) in the rat to study this phenomenon. Herein, we report our experience with LIT using a single donor (SD) or multiple donors (MD). Large volumes of fluid were required to prevent a drop in the mean arterial pressure during the anhepatic phase in the SD recipients. Many of the SD recipients died of intraoperative hypovolemic shock (57%). The MD recipients had a shorter anhepatic time (12 +/- 1 minutes vs. 17 +/- 2 minutes; P less than 0.01) and a shorter warm intestinal ischemia time (15 +/- 1 minutes vs. 32 +/- 2 minutes; P less than 0.01). Operative mortality rates were much lower in the MD recipients (10% vs. 68%; P less than 0.01). The long-term survival rate using MD was 71% at 1 month. Graft function was normal in the long-term survivors. LIT with MD provides a good model to study the immunological effects of multivisceral grafting.
同时进行肝脏移植可能会降低肠道移植排斥反应的风险。我们最近在大鼠中开发了两种新的肝/肠联合移植（LIT）模型来研究这一现象。在此，我们报告我们使用单一供体（SD）或多个供体（MD）进行LIT的经验。在SD受体的无肝期，需要大量液体来防止平均动脉压下降。许多SD受体死于术中低血容量性休克（57%）。MD受体的无肝时间较短（12±1分钟对17±2分钟；P<0.01），热缺血肠时间较短（15±1分钟对32±2分钟；P<0.01）。MD受体的手术死亡率要低得多（10%对68%；P<0.01）。使用MD的长期生存率在1个月时为71%。长期存活者的移植物功能正常。使用MD的LIT为研究多脏器移植的免疫效应提供了一个良好的模型。
