Trepicchio W L, Krontiris T G
Department of Medicine (Hematology/Oncology), New England Medical Center Hospitals, Boston, MA.
Nucleic Acids Res. 1992 May 25;20(10):2427-34. doi: 10.1093/nar/20.10.2427.
The 28 base pair repeat unit of a minisatellite 1000 bp downstream from the human HRAS1 gene (VTRHRAS1) bound four proteins (p45, p50, p72 and p85) in nuclear extracts from a variety of human cell lines which were indistinguishable from several members of the rel/NF-kappa B family of transcriptional regulatory factors. VTRHRAS1 bound the constitutively expressed, but not the inducible, forms of these proteins. Analysis of partially purified binding factors from different cell lines demonstrated qualitative differences in the p50 subunit; phosphocellulose fractionation also revealed considerable heterogeneity in the p72 and p85 subunits. These results suggest the possibility that the HRAS1 minisatellite, in serving as a tandem array of rel/NF-kappa B binding sites, may function in the transcriptional regulation of HRAS1 and nearby genes.
位于人类HRAS1基因下游1000 bp处的一个小卫星的28个碱基对重复单元（VTRHRAS1），在多种人类细胞系的核提取物中结合了四种蛋白质（p45、p50、p72和p85），这些蛋白质与转录调节因子rel/NF-κB家族的几个成员无法区分。VTRHRAS1结合这些蛋白质的组成型表达形式，但不结合诱导型表达形式。对来自不同细胞系的部分纯化结合因子的分析表明，p50亚基存在质的差异；磷酸纤维素分级分离还揭示了p72和p85亚基存在相当大的异质性。这些结果表明，HRAS1小卫星作为rel/NF-κB结合位点的串联阵列，可能在HRAS1和附近基因的转录调控中发挥作用。
