Bhattacharya Sayanti, Subramanian Mahesh, Roychowdhury Susri, Bauri Ajay K, Kamat Jaya P, Chattopadhyay Subrata, Bandyopadhyay Sandip Kumar
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic medical Sciences, Kolkata, India.
J Radiat Res. 2005 Jun;46(2):165-71. doi: 10.1269/jrr.46.165.
The radioprotective activity of Piper betel ethanolic extract (PE) has been studied using rat liver mitochondria and pBR 322 plasmid DNA as two model in vitro systems. The extract effectively prevented gamma-ray induced lipid peroxidation as assessed by measuring thiobarbituric acid reactive substrates, lipid hydroperoxide and conjugated diene. Likewise, it prevented radiation-induced DNA strand breaks in a concentration dependent manner. The radioprotective activity of PE could be attributed to its hydroxyl and superoxide radicals scavenging property along with its lymphoproliferative activity. The radical scavenging capacity of PE was primarily due to its constituent phenolics, which were isolated and identified as chevibetol and allyl pyrocatechol.
已使用大鼠肝线粒体和pBR 322质粒DNA作为两种体外模型系统研究了蒌叶乙醇提取物（PE）的辐射防护活性。通过测量硫代巴比妥酸反应性底物、脂质氢过氧化物和共轭二烯来评估，该提取物有效预防了γ射线诱导的脂质过氧化。同样，它以浓度依赖的方式预防了辐射诱导的DNA链断裂。PE的辐射防护活性可归因于其清除羟基和超氧阴离子自由基的特性以及其淋巴细胞增殖活性。PE的自由基清除能力主要归因于其所含的酚类物质，这些酚类物质被分离并鉴定为蛇床子醇和烯丙基邻苯二酚。
