Gérard N, Syed V, Jégou B
GERM, INSERM CJF 91-04, Université de Rennes I, Bretagne, France.
Biochem Biophys Res Commun. 1992 May 29;185(1):154-61. doi: 10.1016/s0006-291x(05)80969-6.
Several recent studies indicate that interleukin-1 (IL-1) may be one of the major regulators of spermatogenesis. In the present work, the effects of various agents on rat Sertoli cell IL-1 production were investigated in vitro at different ages. In control cultures the IL-1 production by Sertoli cells from immature rats (20 days) was barely detectable; it markedly increased with the advancing age of the donors (35 and 45 days). Lipopolysaccharide and latex beads, two inducers of monocyte-macrophage IL-1 production, were able to stimulate the release of IL-1 by Sertoli cells at all ages studied; a decrease in the relative response to these inducers was noted as the age of the Sertoli cell donors increased. Under all the experimental conditions tested it appeared that the IL-1 produced was IL-1 alpha, not IL-1 beta. Whereas pachytene spermatocytes and early spermatids had no effect on IL-1 alpha production, residual bodies/cytoplasts from elongated spermatids dramatically stimulated Sertoli cell IL-1 production. In addition, FSH had no effect on IL-1 alpha levels. It is concluded that Sertoli cell IL-1 secretion can be stimulated in vitro by well known monocyte-macrophage activators. Furthermore, Sertoli cell IL-1 production is most probably crucially dependent on the phagocytosis of residual bodies in vivo.
最近的几项研究表明，白细胞介素-1（IL-1）可能是精子发生的主要调节因子之一。在本研究中，研究了不同年龄段各种因素对大鼠支持细胞IL-1产生的影响。在对照培养中，未成熟大鼠（20天）支持细胞产生的IL-1几乎检测不到；随着供体年龄的增加（35天和45天），其显著增加。脂多糖和乳胶珠是单核巨噬细胞IL-1产生的两种诱导剂，在所有研究的年龄段都能刺激支持细胞释放IL-1；随着支持细胞供体年龄的增加，对这些诱导剂的相对反应降低。在所有测试的实验条件下，所产生的IL-1似乎是IL-1α，而不是IL-1β。粗线期精母细胞和早期精子细胞对IL-1α的产生没有影响，而延长型精子细胞的残余体/胞质体显著刺激支持细胞IL-1的产生。此外，促卵泡激素对IL-1α水平没有影响。结论是，众所周知的单核巨噬细胞激活剂可在体外刺激支持细胞IL-1的分泌。此外，支持细胞IL-1的产生很可能在体内关键依赖于残余体的吞噬作用。
