Namba A
Biomedical Research Center, Olympus Optical Co. Ltd, Tokyo, Japan.
Front Med Biol Eng. 1992;4(1):47-54.
Presented here is a new method of optically detecting agglutination reactions through counting the number of level-crossings of light intensity fluctuations scattered by coated carrier particles. The theory behind this technique is that the number of level-crossings decreases as the size of aggregated particles and, accordingly, the antigen concentration increases. The antigen used in this experiment is immunoglobulin E (IgE). The crossing level is fixed at the average intensity of the scattered light. The detectable range is from 17 to 1000 IU/ml under this condition. The incubation time is 15 min. The coefficient of variations (CVs) of the number of level-crossings is reasonably low, between 0.382 and 3.05% (n = 5). Through simple data processing involving comparison of the data with the crossing level and counting the number of level-crossings, it is possible to determine the antigen concentration on a real time basis.
本文介绍了一种通过对包被载体颗粒散射的光强波动进行电平交叉计数来光学检测凝集反应的新方法。该技术背后的理论是，随着聚集颗粒尺寸的增加以及相应地抗原浓度的增加，电平交叉的数量会减少。本实验中使用的抗原是免疫球蛋白E（IgE）。交叉电平固定为散射光的平均强度。在此条件下，可检测范围为17至1000 IU/ml。孵育时间为15分钟。电平交叉数量的变异系数（CV）相当低，在0.382%至3.05%之间（n = 5）。通过简单的数据处理，包括将数据与交叉电平进行比较并计算电平交叉的数量，可以实时确定抗原浓度。
