Poncelet S, Lavenne E, Limet J N, Collet-Cassart D
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain.
J Clin Chem Clin Biochem. 1990 Mar;28(3):181-3.
Latex particles coated with F(ab)'2 fragments of anti-protein C IgG antibodies are agglutinated by protein C, and the quantity of particles agglutinated is proportional to the concentration of protein C. The reaction can be quantitated by optical particle counting. Based on this system, we designed an immunoassay for protein C. Precision measured at low, medium and high levels of protein C varied from 3.3% to 13.7%. Specificity was evaluated by dilution recovery. A correlation coefficient of r = 0.959 was found when the new method was compared with a chromogenic technique on 131 plasmas.
用抗蛋白C IgG抗体的F(ab)'2片段包被的乳胶颗粒可被蛋白C凝集，凝集的颗粒数量与蛋白C的浓度成正比。该反应可用光学颗粒计数法定量。基于此系统，我们设计了一种蛋白C免疫测定法。在蛋白C的低、中、高浓度水平下测得的精密度在3.3%至13.7%之间。通过稀释回收率评估特异性。在131份血浆样本上，将新方法与发色法进行比较时，发现相关系数r = 0.959。
