Ramos J J, Marca C, Ferrer L M, Loste A, Cebrián L M
Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, C/Miguel Servet 177, 50013 Zaragoza, Spain.
Res Vet Sci. 2006 Feb;80(1):11-6. doi: 10.1016/j.rvsc.2005.04.009. Epub 2005 Jul 5.
A study was made to investigate faecal thiaminase and the thiamine-related biochemical changes in apparently normal replacement ewes with a feed change, after the initiation without adaptation to the new pasture. Twenty-four female ewes were divided into two groups. Group A was managed in a system based on pasture and was compared with group B system based on a diet of concentrate and straw until moving to pasture 9 weeks after. Blood samples for lactate, pyruvate and erythrocyte transketolase activity determinations and faeces for thiaminase estimation were evaluated chronologically. At the end of a 126 days experimental period, live weights of groups were similar. We confirmed that clinically normal sheep may have thiaminase activity in the faeces and concluded that the thiaminase release increased during the diet changes, from concentrate to pasture, and that their continued excretion could develop some degree of thiamine deficiency.
进行了一项研究，以调查在未适应新牧场就开始更换饲料后，表面正常的替代母羊粪便中的硫胺素酶以及与硫胺素相关的生化变化。24只母羊被分为两组。A组采用基于牧场的饲养系统，并与B组基于精饲料和稻草的饲养系统进行比较，直到9周后转移到牧场。按时间顺序对用于测定乳酸、丙酮酸和红细胞转酮醇酶活性的血样以及用于估计硫胺素酶的粪便进行评估。在126天的实验期结束时，两组的活体重相似。我们证实临床正常的绵羊粪便中可能存在硫胺素酶活性，并得出结论，在从精饲料到牧场的饮食变化过程中，硫胺素酶的释放增加，其持续排泄可能会导致一定程度的硫胺素缺乏。
