Castan Stéphane, Villard Claude, Jakob Stefan, Puigserver Antoine, Ajandouz El Hassan
Institut Méditerranéen de Recherche en Nutrition, case 342, INRA-UMR 1111, Faculté des Sciences et Techniques de Saint-Jérôme, Avenue Escadrille Normandie Niemen, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):339-46. doi: 10.1016/j.jchromb.2005.06.028.
This report improves analytical procedures to investigate the behaviour of the two Vitamin E forms, alpha-tocopherol (Tol) and alpha-tocopheryl acetate (Tac), in model systems mimicking the intestinal medium. We describe how to prepare mixed micelles as vehicle for Tac and Tol and the HPLC method for their quantification in the micelles. Tac and Tol were extracted using ethanol-hexane-drying procedure, whereas the separation and detection were performed in methanol and by UV method, respectively. Both compounds were eluted in less than 4 min. In the range between 1.7 microM and 54 microM of Tac or Tol in the micelles, their recovery were 89% and 81%, respectively, with correlation coefficient over 0.99 and R.S.D. of less than 7.2% in all cases. Limits of detection and quantification for Tac and Tol in mixed micelles ranged between 1 microM and 2 microM and between 3 microM and 5 microM, respectively. The behaviours of Tac and Tol were quite different during the extraction procedure and both were influenced by the vitamin concentration and the relative volume of organic solvents.
本报告改进了分析程序，以研究两种维生素E形式，即α-生育酚（Tol）和α-生育酚乙酸酯（Tac）在模拟肠道介质的模型系统中的行为。我们描述了如何制备混合胶束作为Tac和Tol的载体，以及用于在胶束中对它们进行定量的HPLC方法。Tac和Tol采用乙醇 - 己烷干燥程序进行提取，而分离和检测分别在甲醇中进行并采用紫外法。两种化合物均在不到4分钟内洗脱。在胶束中Tac或Tol浓度介于1.7 microM至54 microM之间时，它们的回收率分别为89%和81%，所有情况下相关系数均超过0.99且相对标准偏差小于7.2%。混合胶束中Tac和Tol的检测限和定量限分别介于1 microM至2 microM和3 microM至5 microM之间。在提取过程中，Tac和Tol的行为差异很大，并且两者均受维生素浓度和有机溶剂相对体积的影响。
