Chang W P, Little J B
Laboratory of Radiobiology, Harvard University School of Public Health, Boston, MA 02115.
Carcinogenesis. 1992 Jun;13(6):923-8. doi: 10.1093/carcin/13.6.923.
Residual damage manifested as reduced cloning efficiency was observed in many of the cloned progeny of Chinese hamster ovary (CHO) cells and human carcinoma SQ-20B cells surviving X-irradiation. This stable phenotype, which we have termed delayed reproductive death, persisted for greater than 50 generations of cell replication post-irradiation. Clones showing this phenotype were aneuploid, and formed colonies with a high proportion of giant cells. By somatic cell hybridization of CHO clones, the delayed reproductive death phenotype was found to be a dominant trait; the cloning efficiency of hybrid clones was persistently depressed, as compared with that of control hybrid cells. These results suggest that delayed reproductive death represents a specific cellular response that may persist in some of the progeny of mammalian cells for long periods after X-irradiation.
在经受X射线照射后存活的中国仓鼠卵巢（CHO）细胞和人SQ-20B癌细胞系的许多克隆后代中，观察到了以克隆效率降低为表现形式的残留损伤。这种稳定的表型，我们称之为延迟性生殖死亡，在照射后的细胞复制超过50代后仍然存在。表现出这种表型的克隆是非整倍体，并且形成的集落中有高比例的巨细胞。通过CHO克隆的体细胞杂交发现，延迟性生殖死亡表型是一个显性性状；与对照杂交细胞相比，杂交克隆的克隆效率持续降低。这些结果表明，延迟性生殖死亡代表了一种特定的细胞反应，在X射线照射后的很长一段时间内，这种反应可能会在哺乳动物细胞的一些后代中持续存在。
