Beda Nataliya, Nedospasov Andrei
Nitric Oxide. 2005 Sep;13(2):93-7. doi: 10.1016/j.niox.2005.05.002.
Miranda et al. have developed a method for simultaneous evaluation of nitrate and nitrite concentrations using reduction of nitrate by vanadium(III) combined with detection by the acidic Griess reaction [K.M. Miranda, M.G. Espey, D.A. Wink, A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite, Nitric Oxide 5 (2001) 62-71]. The sensitivity of the nitrate assay decline if the mixture analyzed contains a large excess of nitrite relative to nitrate, for instance, in the case of oxidation products of nitric oxide (NO) in aerated solutions, or in sweat. By this reason nitrite should be removed before the nitrate assay, if [NO2-]>>[NO3-]. Here we lay out an improved method allowing the above limitation to be erased, using sulfamic acid for nitrite removal. We also describe some modifications that enhance the reproducibility of the assay.
米兰达等人开发了一种同时评估硝酸盐和亚硝酸盐浓度的方法，该方法利用钒（III）还原硝酸盐，并结合酸性格里斯反应进行检测[K.M. 米兰达、M.G. 埃斯佩、D.A. 温克，一种快速、简单的同时检测硝酸盐和亚硝酸盐的分光光度法，《一氧化氮》5（2001年）62 - 71页]。如果被分析的混合物中亚硝酸盐相对于硝酸盐过量很多，例如在曝气溶液或汗液中一氧化氮（NO）的氧化产物的情况下，硝酸盐测定的灵敏度会下降。因此，如果[NO₂⁻] >> [NO₃⁻]，在进行硝酸盐测定之前应去除亚硝酸盐。在此，我们阐述一种改进的方法，使用氨基磺酸去除亚硝酸盐，从而消除上述限制。我们还描述了一些提高测定重现性的改进措施。
