Happel Nicole, Schulze Ekkehard, Doenecke Detlef
Institute for Biochemistry and Molecular Cell Biology, University of Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany.
Biol Chem. 2005 Jun;386(6):541-51. doi: 10.1515/BC.2005.064.
The members of the H1 histone family can be classified into three groups, which are the main class subtypes expressed in somatic cells, the developmental- and tissue-specific subtypes, and the replacement subtype H1(o). Until now, the subtype H1x was not classified, since it has not yet been thoroughly examined. The results of this study show that H1x shares similarities but also exhibits slight differences in its biochemical behaviour in comparison to the main class H1 histones. In HeLa cells it is located in the nucleus and partially associated with nucleosomes. Nevertheless, it is, like H1(o), mainly located in chromatin regions that are not affected by micrococcal nuclease digestion. Further common features of H1x and the replacement histone H1(o) are that the genes of both subtypes are solitarily located and give rise to polyadenylated mRNA. However, comparison of the inducibility of their expression revealed that their genes are regulated differentially.
H1组蛋白家族的成员可分为三类，即体细胞中表达的主要类别亚型、发育和组织特异性亚型以及替代亚型H1(o)。到目前为止，H1x亚型尚未分类，因为尚未对其进行全面研究。本研究结果表明，与主要类别H1组蛋白相比，H1x在生化行为上既有相似之处，也有细微差异。在HeLa细胞中，它位于细胞核内，部分与核小体相关。然而，与H1(o)一样，它主要位于不受微球菌核酸酶消化影响的染色质区域。H1x和替代组蛋白H1(o)的进一步共同特征是，这两种亚型的基因都是单独定位的，并产生多聚腺苷酸化的mRNA。然而，对它们表达诱导性的比较表明，它们的基因受到不同的调控。
