Revel Ariel, Haimov-Kochman Ronit, Porat Anat, Lewin Aby, Simon Alex, Laufer Neri, Gino Hagar, Meirow Dror
IVF Unit, Hadassah University Hospital, Jerusalem, Israel.
Fertil Steril. 2005 Jul;84(1):118-22. doi: 10.1016/j.fertnstert.2005.01.121.
To describe the success rate of intracytoplasmic sperm injection (ICSI) using thawed cryopreserved sperm in male cancer patients.
Retrospective consecutive study.
IVF unit in a tertiary university hospital.
PATIENT(S): Twenty-one couples treated by ICSI using the frozen-thawed sperm of husbands treated for cancer.
INTERVENTION(S): Follow-up of all treatments by ICSI.
MAIN OUTCOME MEASURE(S): Pregnancy rate, sperm count, and fertilization rate.
RESULT(S): Male patients being treated for cancer froze between one and 29 sperm samples. Post-thaw sperm count ranged from 1 x 10(5) to 106 x 10(6). The mean fertilization rate by ICSI was 60% (range, 33%-100%), and the pregnancy rate was 42%. Twenty-six pregnancies were obtained resulting in 23 children (13 singleton and 10 twins) and eight spontaneous abortions (31%). Delivery was obtained in 12 of the 21 treated couples (57%). The lowest total motile sperm count that resulted in a pregnancy was 1 x 10(5).
CONCLUSION(S): Male cancer patients should be encouraged to freeze numerous sperm samples even when sperm count and motility are poor. In these cases, ICSI is a powerful technique compared with intrauterine injection since thawed sperm samples with poor parameters can produce relatively high fertilization rates resulting in normal pregnancies and deliveries. The possibility to repeat treatments even in the face of a limited number of sperm samples appears to be of importance.
描述在男性癌症患者中使用解冻的冷冻保存精子进行卵胞浆内单精子注射（ICSI）的成功率。
回顾性连续研究。
一所三级大学医院的体外受精单元。
21对夫妇接受了ICSI治疗，使用的是患癌症丈夫的冷冻解冻精子。
对所有ICSI治疗进行随访。
妊娠率、精子计数和受精率。
正在接受癌症治疗的男性患者冷冻了1至29份精子样本。解冻后的精子计数范围为1×10⁵至106×10⁶。ICSI的平均受精率为60%（范围为33%-100%），妊娠率为42%。共获得26次妊娠，产下23名儿童（13名单胎和10对双胞胎），8例自然流产（31%）。21对接受治疗的夫妇中有12对分娩（57%）。导致妊娠的最低总活动精子计数为1×10⁵。
应鼓励男性癌症患者即使在精子计数和活力较差时也冷冻大量精子样本。在这些情况下，与宫腔内注射相比，ICSI是一种有效的技术，因为参数较差的解冻精子样本可产生相对较高的受精率，从而实现正常妊娠和分娩。即使面对有限数量的精子样本仍有可能重复治疗，这一点似乎很重要。
