Yoo Je Young, Kim Jong Min, Seo Kwang Su, Jeong Yong Kyun, Lee Hai Bang, Khang Gilson
Department of Advanced Organic Materials Engineering, Chonbuk National University, 664-14, Duckjin, Jeonju 561-756, Korea.
Biomed Mater Eng. 2005;15(4):279-88.
The aim of this study was to detect the amount of lactic acid (LA) and glycolic acid (GA) in poly(D,L-lactide-co-glycolide) (PLGA) by development a simple HPLC method and to determine the pH of media, which can influence on degradation of PLGA and drug release. Analysis of in vitro degradation behavior of PLGA with two different molecular weights as 8000 and 33,000 g/mol were performed in various media conditions (pH 3.0, 5.0, 7.0, and 9.0 of PBS and distilled water (approx. pH 5.8)). Also, effect of some additives on PLGA degradation was also investigated in pH 7.0 of PBS. GA and LA were easily detected by a simple HPLC method (retention time: 6.5 min and 10.2 min, respectively). The result showed that GA was released larger amount than that of LA considering the initial sample weight of polymers, due to the higher hydrophilic property. In the lower pH of media conditions, the PLGA was faster degraded generally. The presence of various additives, moreover, affected decrease of pH and slight acceleration of LA and GA detection.
本研究的目的是通过开发一种简单的高效液相色谱法来检测聚（D，L-丙交酯-共-乙交酯）（PLGA）中乳酸（LA）和乙醇酸（GA）的含量，并确定可影响PLGA降解和药物释放的介质pH值。在各种介质条件下（pH值为3.0、5.0、7.0和9.0的磷酸盐缓冲盐水（PBS）以及蒸馏水（约pH 5.8））对两种不同分子量（8000和33,000 g/mol）的PLGA进行体外降解行为分析。此外，还在pH 7.0的PBS中研究了一些添加剂对PLGA降解的影响。通过一种简单的高效液相色谱法（保留时间分别为6.5分钟和10.2分钟）可轻松检测到GA和LA。结果表明，考虑到聚合物的初始样品重量，由于GA具有更高的亲水性，其释放量比LA大。在较低pH值的介质条件下，PLGA通常降解得更快。此外，各种添加剂的存在会影响pH值的降低以及LA和GA检测的轻微加速。
