Desmadryl G, Dechesne C J
INSERM U-254, USTL, Montpellier, France.
Exp Brain Res. 1992;89(1):105-8. doi: 10.1007/BF00229006.
Immunohistochemical investigations with calretinin, a neuronal calcium binding protein, were made in the vestibular end organs of five guinea pigs and one chinchilla. A specific pattern of immunoreactivity of afferent nerve fibers was found. Immunostaining was restricted to thick fibers innervating the apex of the cristae or the striola of the utricular macula. A study of serial sections revealed that the stained afferents gave rise to calyx endings, but not to collaterals containing bouton endings. The results are consistent with the conclusion that, of the three classes of fibers defined by Fernández et al. (1988, 1990), only calyx units are calretinin immunoreactive. A count of the number of labelled fibers in the chinchilla crista suggests that the entire population of calyx units is immunoreactive. The conclusion is surprising since the physiology of calyx units does not differ qualitatively from that of other afferents (Baird et al. 1988; Goldberg et al. 1990). The presence of this protein in the calyx neurons may be related to specific post-synaptic functions of this type of afferents.
利用神经元钙结合蛋白钙视网膜蛋白对5只豚鼠和1只毛丝鼠的前庭终器进行了免疫组织化学研究。发现传入神经纤维存在特定的免疫反应模式。免疫染色仅限于支配嵴顶或椭圆囊斑纹区的粗纤维。对连续切片的研究表明，染色的传入纤维形成了花萼样终末，但没有形成含有纽扣样终末的侧支。这些结果与以下结论一致：在费尔南德斯等人（1988年、1990年）定义的三类纤维中，只有花萼样单位具有钙视网膜蛋白免疫反应性。对毛丝鼠嵴中标记纤维数量的计数表明，整个花萼样单位群体都具有免疫反应性。这一结论令人惊讶，因为花萼样单位的生理学与其他传入纤维在性质上并无不同（贝尔德等人，1988年；戈德堡等人，1990年）。这种蛋白质在花萼样神经元中的存在可能与这类传入纤维的特定突触后功能有关。
