Inoue Ken-Ichiro, Takano Hirohisa, Shiga Akira, Fujita Yoji, Makino Hiroaki, Yanagisawa Rie, Ichinose Takamichi, Kato Yoji, Yamada Tomozo, Yoshikawa Toshikazu
Inhalation Toxicology & Pathophysiology Research Team, National Institute for Environmental Studies, Ibaraki, Japan.
Int J Mol Med. 2005 Aug;16(2):315-9.
Perilla leaf extract is known to have anti-inflammatory properties. Recently, we have demonstrated that rosmarinic acid, a polyphenolic liquid component in perilla, inhibits the allergic airway inflammation induced by house dust mites (HDMs) in vivo. The purpose of this study was to evaluate the effects of intratracheal (i.t.) exposure to volatile constituents of a rosemary extract (VR), gaseous components in perilla, on a murine model of allergic asthma induced by HDM. C3H/HeN mice were treated 7 times weekly with i.t. exposure. The HDM allergen challenge elicited a pulmonary eosinophilic inflammation accompanied by an increase in the lung expression of interleukin (IL)-5, IL-13, and eotaxin. VR inhibited increases in the number of eosinophils, neutrophils, and mononuclear cells around the airways and those in the bronchoalveolar lavage fluid. VR exposure also significantly suppressed the expression of IL-13 enhanced by HDM allergen. These results suggest that i.t. exposure to VR can, at least partially, prevent allergic airway inflammation induced by HDM. The preventive effect is associated with inhibition of the enhanced local expression of IL-13.
紫苏叶提取物已知具有抗炎特性。最近，我们已经证明，迷迭香酸，紫苏中的一种多酚类液体成分，在体内可抑制屋尘螨（HDM）诱导的过敏性气道炎症。本研究的目的是评估气管内（i.t.）暴露于迷迭香提取物的挥发性成分（VR），即紫苏中的气态成分，对HDM诱导的过敏性哮喘小鼠模型的影响。C3H/HeN小鼠每周接受7次i.t.暴露治疗。HDM变应原激发引发了肺部嗜酸性粒细胞炎症，同时白细胞介素（IL）-5、IL-13和嗜酸性粒细胞趋化因子的肺表达增加。VR抑制了气道周围及支气管肺泡灌洗液中嗜酸性粒细胞、中性粒细胞和单核细胞数量的增加。VR暴露还显著抑制了HDM变应原增强的IL-13表达。这些结果表明，i.t.暴露于VR至少可以部分预防HDM诱导的过敏性气道炎症。预防作用与抑制IL-13局部表达增强有关。
