Kim Shin-Hee, Wei Cheng-I, An Haejung
Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
J Food Prot. 2005 Jul;68(7):1408-13. doi: 10.4315/0362-028x-68.7.1408.
Sixty-four multidrug-resistant isolates of Proteus mirabilis were collected from retail meat products in Oklahoma. The isolates showed four different patterns of antibiotic resistance based on their resistant phenotype and genotypes. Most of these isolates were resistant to ampicillin, tetracycline, gentamycin, and kanamycin. Class 1 integrons were detected as a common carrier of the antibiotic-resistant genes, such as aadA1, aadB, and aadA2. A few isolates (9%) contained class 2 integrons with three gene cassettes included: dhfr1, sat1, and aadA1. These isolates were even resistant to nalidixic acid due to mutations in gyrA and parC. All ampicillin-resistant isolates contained blaTEM-1. Plasmids that contained class 1 or 2 integrons and blaTEM-1 were able to be transferred from P. mirabilis isolates into Escherichia coli by conjugation, indicating that conjugal transfer could contribute to the dissemination of antibiotic resistance genes between the Enterobacteriaceae species.
从俄克拉荷马州的零售肉类产品中收集了64株奇异变形杆菌多重耐药菌株。根据其耐药表型和基因型，这些菌株呈现出四种不同的抗生素耐药模式。这些菌株大多对氨苄西林、四环素、庆大霉素和卡那霉素耐药。1类整合子被检测为抗生素耐药基因（如aadA1、aadB和aadA2）的常见载体。少数菌株（9%）含有2类整合子，其中包括三个基因盒：dhfr1、sat1和aadA1。由于gyrA和parC发生突变，这些菌株甚至对萘啶酸耐药。所有对氨苄西林耐药的菌株都含有blaTEM-1。含有1类或2类整合子以及blaTEM-1的质粒能够通过接合作用从奇异变形杆菌菌株转移到大肠杆菌中，这表明接合转移可能有助于抗生素耐药基因在肠杆菌科物种之间传播。
