Akane A, Seki S, Shiono H, Nakamura H, Hasegawa M, Kagawa M, Matsubara K, Nakahori Y, Nagafuchi S, Nakagome Y
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Forensic Sci Int. 1992 Jan;52(2):143-8. doi: 10.1016/0379-0738(92)90102-3.
Sex determination by polymerase chain reaction (PCR) analysis of the X-Y homologous amelogenin gene is highly reliable since the detection of an X-specific amplified fragment validates the procedure. Previously, we reported that 250 ng of template DNA are required for sex determination by this method. We report here a refinement of the technique to include dual PCR. Dual PCR using two sets of primers results in the detection of X- and Y-specific amplified fragments from as little as 0.005 ng of template DNA. This is a powerful technique for the analysis of trace forensic samples and its application is discussed.
通过聚合酶链反应（PCR）分析X-Y同源牙釉蛋白基因来确定性别是高度可靠的，因为检测到X特异性扩增片段就验证了该程序。此前，我们报道过用这种方法确定性别需要250 ng模板DNA。我们在此报告该技术的改进，包括双重PCR。使用两组引物的双重PCR能够从低至0.005 ng的模板DNA中检测到X和Y特异性扩增片段。这是一种用于分析微量法医样本的强大技术，并对其应用进行了讨论。
